| 1.1 | Can the Circle Be Unbroken | Mother Maybelle Carter, A.P. Carter, Sara Carter | 3:07 |
| 1.2 | Blue Yodel No. 8 (Mule Skinner Blues) | Jimmie Rodgers | 2:57 |
| 1.3 | Barbara Allen | Bradley Kincaid | 2:33 |
| 1.4 | I’ll Fly Away | James & Martha Carson | 2:23 |
| 1.5 | If the River Was Whiskey | Charlie Poole | 3:08 |
| 1.6 | Fox Chase | DeFord Bailey | 1:09 |
| 1.7 | Blue Yodel No. 9 (Standin’ on the Corner) | Jimmie Rodgers | 2:37 |
| 1.8 | Wildwood Flower | Sara Carter | 3:09 |
| 1.9 | In the Jailhouse Now | Jimmie Rodgers | 3:18 |
| 1.10 | Comin’ Round the Mountain | Uncle Dave Macon, Sam McGee | 3:07 |
| 1.11 | Pretty Polly | The Coon Creek Girls | 2:48 |
| 1.12 | T.B. Blues | Jimmie Rodgers | 2:59 |
| 1.13 | Mountain Dew | Grandpa Jones and his Granchildren | 2:43 |
| 1.14 | Home on the Range | Gene Autry | 2:38 |
| 1.15 | I Want to Be a Cowboy’s Sweetheart | Rubye Blevins | 3:07 |
| 1.16 | Tumbling Tumbleweeds (single version) | Sons of the Pioneers | 3:12 |
| 1.17 | Keep on the Sunny Side / I’m Thinking Tonight of My Blue Eyes | The Carter Family | 2:06 |
| 1.18 | The Great Speckled Bird | Roy Acuff | 2:40 |
| 1.19 | Whoa Babe | Leon McAuliffe | 2:34 |
| 1.20 | New San Antonio Rose | Tommy Duncan | 2:36 |
| 1.21 | Wabash Cannonball | Roy Acuff | 2:35 |
| 1.22 | Mule Skinner Blues (Blue Yodel #8) | Bill Monroe and the Bluegrass Boys | 2:58 |
| 2.1 | Honky Tonkin’ | Hank Williams with The Drifting Cowboys | 2:43 |
| 2.2 | It’s Mighty Dark to Travel | Bill Monroe and the Bluegrass Boys | 2:52 |
| 2.3 | New Mule Skinner Blues | The Maddox Brothers & Rose | 3:07 |
| 2.4 | I’ll Hold You in My Heart (Till I Can Hold You in My Arms) | Eddy Arnold | 2:42 |
| 2.5 | Foggy Mountain Breakdown | Lester Flatt & Earl Scruggs with The Foggy Mountain Boys | 2:40 |
| 2.6 | Molly and Tenbrook | The Stanley Brothers | 2:33 |
| 2.7 | Lovesick Blues (single version) | Hank Williams | 2:43 |
| 2.8 | I Saw the Light | Hank Williams | 2:13 |
| 2.9 | Hey, Good Lookin’ | Hank Williams | 3:19 |
| 2.10 | It Wasn’t God Who Made Honky Tonk Angels | Kitty Wells | 2:30 |
| 2.11 | I’m So Lonesome I Could Cry | Hank Williams with The Drifting Cowboys | 2:45 |
| 2.12 | Jambalaya (single version) | Brenda Lee | 2:08 |
| 2.13 | New Step It Up and Go | Don Maddox | 2:24 |
| 2.14 | I Walk the Line | Johnny Cash | 2:44 |
| 2.15 | Crazy Arms | Ray Price | 2:31 |
| 2.16 | Bye, Bye Love | The Everly Brothers | 2:17 |
| 2.17 | The Long Black Veil | Lefty Frizzell | 3:08 |
| 2.18 | El Paso | Marty Robbins | 4:38 |
| 2.19 | Night Life | Ray Price | 4:45 |
| 2.20 | Hello Walls | Faron Young | 2:24 |
| 2.21 | I Fall to Pieces (single version) | Patsy Cline | 2:47 |
| 2.22 | Ring of Fire | Johnny Cash, The Carter Family | 2:35 |
| 2.23 | Crazy | Patsy Cline | 2:42 |
| 2.24 | I Can’t Stop Loving You | Ray Charles | 4:13 |
| 3.1 | Dang Me | Roger Miller | 1:49 |
| 3.2 | I’ve Got a Tiger by the Tail | Buck Owens, Don Rich | 2:12 |
| 3.3 | Don’t Come Home a Drinkin’ (with Lovin’ on Your Mind) | Loretta Lynn, The Jordanaires | 2:07 |
| 3.4 | Coal Miner’s Daughter | Loretta Lynn, The Jordanaires | 2:58 |
| 3.5 | Kiss an Angel Good Mornin’ | Charley Pride | 2:02 |
| 3.6 | Hungry Eyes | Merle Haggard and The Strangers | 3:26 |
| 3.7 | Mama Tried | Glen Campbell, Merle Haggard, Bonnie Owens | 2:11 |
| 3.8 | Harper Valley P.T.A. | Jeannie C. Riley | 3:13 |
| 3.9 | Don’t Touch Me | Jeannie Seely | 3:12 |
| 3.10 | Folsom Prison Blues (live at Folsom State Prison, Folsom, CA - January 1968) | Johnny Cash | 2:43 |
| 3.11 | Stand by Your Man | Tammy Wynette | 2:38 |
| 3.12 | She Thinks I Still Care | George Jones | 2:34 |
| 3.13 | You Ain’t Goin’ Nowhere | Roger McGuinn, Chris Hillman | 2:33 |
| 3.14 | Me and Bobby McGee | Kris Kristofferson | 4:22 |
| 3.15 | Help Me Make It Through the Night | Sammi Smith | 2:33 |
| 3.16 | Sunday Mornin’ Comin’ Down | Kris Kristofferson | 4:36 |
| 3.17 | Okie From Muskogee | Merle Haggard | 2:42 |
| 3.18 | Man in Black | Johnny Cash | 2:52 |
| 3.19 | Girl From the North Country | Bob Dylan, Johnny Cash | 3:42 |
| 3.20 | Grand Ole Opry Song | Jimmy Martin, Jeff Hanna, Jimmy Ibbotson, Gary Scruggs, Ray Martin, Les Thompson | 3:11 |
| 3.21 | Will the Circle Be Unbroken | Nitty Gritty Dirt Band | 4:48 |
| 4.1 | Are You Sure Hank Done It This Way | Waylon Jennings | 2:57 |
| 4.2 | Mule Skinner Blues (Blue Yodel No. 8) | Dolly Parton | 3:08 |
| 4.3 | Jolene | Dolly Parton, Hurshel Wiginton, Joe Babcock, Dolores Edgin, June Page | 2:41 |
| 4.4 | I Will Always Love You | Dolly Parton, Hurshel Wiginton, Joe Babcock, Dolores Edgin, June Page | 2:55 |
| 4.5 | We’re Gonna Hold On | The Jordanaires | 2:57 |
| 4.6 | Texas Cookin’ | Guy Clark | 3:27 |
| 4.7 | If I Needed You | Townes Van Zandt | 3:43 |
| 4.8 | I Can’t Stop Loving You | Johnny Rodriguez | 2:57 |
| 4.9 | I’ve Been a Long Time Leaving (But I’ll Be a Long Time Gone) | Waylon Jennings | 2:41 |
| 4.10 | Love Hurts (live) | Gram Parsons with The Fallen Angels | 4:45 |
| 4.11 | Boulder to Birmingham | Emmylou Harris, Herb Pedersen | 3:34 |
| 4.12 | Bluebird Wine | Emmylou Harris, Herb Pedersen, Fayssoux Starling | 3:19 |
| 4.13 | Whiskey River (live at Harrah’s Casino, Lake Tahoe, NV - April 1978) | Willie Nelson | 3:38 |
| 4.14 | Miles and Miles of Texas | Asleep at the Wheel | 3:03 |
| 4.15 | Blue Eyes Crying in the Rain | Willie Nelson | 2:19 |
| 4.16 | A Good Hearted Woman | Waylon Jennings, Willie Nelson | 2:58 |
| 4.17 | Family Tradition | Hank Williams, Jr. | 3:59 |
| 4.18 | Seven Year Ache | Rosanne Cash | 3:15 |
| 4.19 | Pancho and Lefty | Merle Haggard, Willie Nelson, Bobby Wood, Johnny Christopher | 4:46 |
| 4.20 | He Stopped Loving Her Today | George Jones, [unknown] | 3:16 |
| 5.1 | Don’t Get Above Your Raisin’ | Ricky Skaggs | 3:11 |
| 5.2 | On the Road Again (live) | Willie Nelson | 2:33 |
| 5.3 | Amarillo by Morning | George Strait | 2:52 |
| 5.4 | Somebody Should Leave | Reba McEntire | 3:32 |
| 5.5 | Diggin’ Up Bones | Randy Travis | 2:59 |
| 5.6 | Why Not Me | The Judds | 3:30 |
| 5.7 | Honky Tonk Man | Dwight Yoakam, Brantley Kearns | 2:47 |
| 5.8 | Streets of Bakersfield | Dwight Yoakam, Buck Owens | 2:49 |
| 5.9 | Where’ve You Been | Kathy Mattea | 3:45 |
| 5.10 | I’m No Stranger to the Rain | Keith Whitley | 3:34 |
| 5.11 | Go Rest High on That Mountain | Ricky Skaggs, Patty Loveless | 5:14 |
| 5.12 | Guitar Town | Steve Earle | 2:35 |
| 5.13 | She’s in Love With the Boy | Trisha Yearwood, Garth Fundis | 4:05 |
| 5.14 | Tennessee Flat Top Box | Rosanne Cash | 3:09 |
| 5.15 | Get Up John | Emmylou Harris with The Nash Ramblers | 3:01 |
| 5.16 | Uncle Pen | Ricky Skaggs | 2:23 |
| 5.17 | I Still Miss Someone (live) | Rosanne Cash | 2:41 |
| 5.18 | Will the Circle Be Unbroken | Doc Watson, Jimmy Martin, Roy Acuff, Randy Scruggs, Mother Maybelle Carter, Jeff Hanna, Jimmy Ibbotson, Larry Murray, Gary Scruggs, Steve Scruggs, Louise Scruggs, Ray Martin, Gloria Belle, Timmy Martin, Chet Flippo, Les Thompson | 4:47 |