Tracks
| # | Title | Sung by | |
|---|
| 1 | Po Lazarus | James Carter, group of male prisoners | 4:31 |
| 2 | Big Rock Candy Mountain | Harry “Haywire Mac” McClintock | 2:18 |
| 3 | You Are My Sunshine | Norman Blake | 4:28 |
| 4 | Down to the River to Pray | Gillian Welch, Maura O’Connell, Norman Blake, Tim O’Brien, Sam Phillips, David Rawlings, Alison Krauss, Pat Enright, The First Baptist Choir of White House, Tennessee, Dub Cornett, Porter McClister | 2:56 |
| 5 | I Am a Man of Constant Sorrow | Dan Tyminski, Harley Allen, Pat Enright | 3:10 |
| 6 | Hard Time Killing Floor Blues | Chris Thomas King | 2:42 |
| 7 | I Am a Man of Constant Sorrow | Norman Blake | 4:29 |
| 8 | Keep on the Sunny Side | Sharon White, Buck White, Cheryl White | 3:34 |
| 9 | I’ll Fly Away | Gillian Welch, Alison Krauss | 3:58 |
| 10 | Didn’t Leave Nobody but the Baby | Emmylou Harris, Gillian Welch, Alison Krauss | 1:58 |
| 11 | In the Highways | Sarah Peasall, Hannah Peasall, Leah Peasall | 1:36 |
| 12 | I Am Weary (Let Me Rest) | Suzanne Cox, Sidney Cox, Willard Cox | 3:14 |
| 13 | I Am a Man of Constant Sorrow | John Hartford | 2:34 |
| 14 | O Death | Ralph Stanley | 3:20 |
| 15 | In the Jailhouse Now | Pat Enright, Tim Blake Nelson | 3:36 |
| 16 | I Am a Man of Constant Sorrow | Dan Tyminski, Harley Allen, Pat Enright | 4:17 |
| 17 | Indian War Whoop | John Hartford | 1:30 |
| 18 | Lonesome Valley | Isaac Freeman, James Hill, Wilson Waters, Robert Hamlett, Joseph Rice, Nathan Best | 4:07 |
| 19 | Angel Band | Ralph Stanley, Carter Stanley, Curley Lambert, Bill Lowe | 2:16 |
Source: MusicBrainz release group 6fb9820a-99cc-318f-bae4-44992f2b7ffa (CC0). Cover: Cover Art Archive.
O Brother, Where Art Thou?