Tracks
| # | Title | Sung by | |
|---|
| 1 | I'm a Man of Constant Sorrow | The Stanley Brothers | 3:00 |
| 2 | Keep on the Sunny Side | Mother Maybelle Carter, A.P. Carter, Sara Carter | 2:51 |
| 3 | Big Rock Candy Mountain | Harry “Haywire Mac” McClintock | 2:19 |
| 4 | Hard Time Killin' Floor Blues | Skip James | 2:51 |
| 5 | You Are My Sunshine | Jimmie Davis | 2:49 |
| 6 | I'll Fly Away | The Humbard Family | 2:38 |
| 7 | I'm Blue, I'm Lonesome (Blue and Lonesome) | Bill Monroe, Jimmy Martin | 2:52 |
| 8 | Lonesome Valley | Sanctified Group | 3:23 |
| 9 | Indian War Whoop | Hoyt Ming & His Pep-Steppers | 3:13 |
| 10 | In the Jailhouse Now | Jimmie Rodgers | 3:21 |
| 11 | In the Highways | The Carter Family | 1:18 |
| 12 | Sandy Land | Fiddlin' Sam Long | 2:40 |
| 13 | Uncle Pen | Bill Monroe, Jimmy Martin, Joel Price | 2:44 |
| 14 | John the Revelator | Blind Willie Johnson, Willie B. Harris, Angeline Johnson | 3:21 |
| 15 | Way Down the Old Plank Road | Uncle Dave Macon | 3:02 |
| 16 | Blue Yodel No. 8 (Mule Skinner Blues) | Jimmie Rodgers | 3:00 |
| 17 | St. Louis Blues | Bessie Smith | 3:12 |
| 18 | Country Blues | Dock Boggs | 3:00 |
| 19 | Golden Gate Gospel Train | The Golden Gate Jubilee Quartet | 2:37 |
| 20 | The White Dove | Ralph Stanley, Carter Stanley, Darrell "Pee Wee" Lambert | 3:16 |
Source: MusicBrainz release group 6fa7e7cb-69d2-34bc-a177-db0ac7a5aba7 (CC0). Cover: Cover Art Archive.